1. Production of Biotherapeutics
2. Development of Nanobiotics
3. Process Development for the Production of Commercially Important Enzymes.

PhD Students Guided

               2025

1. Kajal Kumari, Ishita Shrestha, Hare Ram Singh & Muthu Kumar Sampath, Different preparation and characterization techniques of lipid nanoparticle and its application as drug carrier.  Vegetos (2025) [SCOPUS, Citescore 2.4] https://doi.org/10.1007/s42535-025-01605-3
2. Kajal Kumari, Hare Ram Singh & Muthu Kumar Sampath, Novel UV-facilitated Green Synthesis of Silver Nanoparticles with Schleichera Oleosa Leaf Extract and its Functionalisation on Lipid Nanoparticles for amoxicillin-silver Conjugated Delivery System against Methicillin Resistant S.aureus Biofilm Reduction. Journal of Cluster Science (2025) 36:228. (SCI, IF 2.7) https://doi.org/10.1007/s10876-025-02948-2
3. Kajal Kumari, Hare Ram Singh1& Muthu Kumar Sampath, Enhanced amoxicillin delivery via artificial intelligence (AI)-based optimized lipid nanoparticles for Helicobacter pylori, Biologia (2025) 80:133-148. (SCI, IF 1.653) https://doi.org/10.1007/s11756-024-01825-z                                                                                                                                                                                                                  2021                                                                                                                                                                             
4. Farkhunda Fatima, Hare Ram Singh, Santosh Kumar Jha, Assessment of antioxidant and cytotoxicity activities against A 549 lung cancer cell line by synthesized reduced graphene oxide nanoparticles mediated by Camellia sinensis, 3 Biotech (2021) 11:494. DOI: 10.1007/s13205-021-03015-z (SCIE, IF 2.8)
5. Pragya Prakash, Sanjeev Chandrayan, Purnima Tiwari, Hare Ram Singh & Santosh Kumar Jha, Development of a downstream process for purification and purity analysis of glutaminase free L-asparaginase using UPLC, DLS-ZP and DSCTGA, Journal of Taibah University for Science, 15:1, (2021), 458-467,  To link to this article: https://doi.org/10.1080/16583655.2021.1984694  (SCI, IF 3.3)
6. Ananya Naha, Santosh Kumar Jha, Hare Ram Singh, Muthu Kumar Sampath. Kinetic modelling and statistical optimization of submerged production of anti-Parkinson’s prodrug L-DOPA by Pseudomonas fluorescens, Preparative Biochemistry and Biotechnology, (2021), https://doi.org/10.1080/10826068.2021.1945624. (SCIE, IF 2.9)
7. Sushil Kumar Shukla, Sneha Pandey, Swastika Saha, Hare Ram Singh, Pradeep Kumar Mishra, Sunil Kumar, Santosh Kumar Jha, Removal of crystal violet by Cu-chitosan nano-biocomposite particles using Box–Behnken design, Journal of Environmental Chemical Engineering 9 (2021), 105847. https://doi.org/10.1016/j.jece.2021.105847 (SCIE, IF 7.7)
8. Pragya Prakash, Hare Ram Singh, Santosh Kumar Jha. Preparation, Characterization and Application of Curcumin-based Polymeric Bio-composite for Efficient Removal of Endotoxins and Bacterial Cells from Therapeutic Preparations. Journal of Biomaterials Science, Polymer Edition, (2021), VOL. 32, NO. 5, 563–580 To link to this article: https://doi.org/10.1080/09205063.2020.1851557 (SCI, IF: 2.69).
9. S. Saha, S.K. Shukla, H.R. Singh, K.K. Pradhan, S.K. Jha. Production and purification of bioflocculants from newly isolated bacterial species: A comparative decolorization study of cationic and anionic textile dyes. Environmental Technology, (2021) VOL. 42, NO. 23, 3663–3674     https://doi.org/10.1080/09593330.2020.1737737. (SCI, IF: 2.8)                                                                2020                                                                                                                  
10. Prakash P, Singh HR, Jha SK. Indicator dye-based screening of glutaminase free L-asparaginase producer and kinetic evaluation of enzyme production process. Preparative biochemistry & biotechnology, (2020), https://doi.org/10.1080/10826068.2020.1737942 (SCIE, IF: 2.9
11. Prakash P, Singh HR & Jha SK. Production, purification and kinetic characterization of glutaminase free anti-leukemic L-asparaginase with low endotoxin level from novel soil isolate. Preparative biochemistry & biotechnology, (2020), VOL. 50, NO. 3, 260–271. To link to this article: https://doi.org/10.1080/10826068.2019.1692221  (SCIE, IF: 2.9)                                                      2019                                                                                                
12. Naha A., Jha S.K, Singh H.R. L-DOPA a Promising Pro-Drug against Parkinson’s Disease: Present and Future Perspective. Research Journal of Biotechnology, 2019; 14 (9), 131-139. (SCOPUS, IF: 1.3
13. Sinha Rati., Singh Hare Ram, Cloning, purification and kinetic characterization of L-asparaginase from Pseudomonas fluorescens. Research Journal of Biotechnology, 2019; 14 (12), 104-110. (SCOPUS, IF: 1.3)

2017

14. Rati Sinha, Hare Ram Singh, Thermokinetic studies of anti-leukemic l-asparaginase of pseudomonas fluorescens , Plant Archives, (2017), 17(2), 1085-1089, doi: 1085-1089 (3883).pdf (plantarchives.org)                                                                                                                                                                                                                                                             2016                                                                                                                                                                                
15. Parasar P, Dubey, A.K., Singh H.R., Jha S.K. Changes in Rheological and Biochemical Properties of Saliva and Semen in Relation to Increasing Body Mass Index (BMI) of Human Subjects. International Journal of Pharmaceutical Sciences Review and Research, (2016); 37(1):122-124. (IF: 0.65)

              2015

16. Perween, Q., Anand, V., Singh, H. R., Jha, S. K., Enhanced Recovery of L-glutaminase by the Optimization of A Three-phase Partitioning System Using the Taguchi Doe Methodology. International Journal Pharmaceutical and Clinical Research. (2015), 7(3): 216-220.)
17. Anand, V., Perween, Q., Sinha, R. K., Singh, H. R., Jha, S. K., Screening of L-glutaminase Producer   and Optimization of Production Process by Taguchi Orthogonal Array. International Journal Pharmaceutical and Clinical Research. (2015), 7(4): 246-251.
18. Jha, S. K., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., A Heuristic Approach for the enhanced recovery of anti-leukemic L-Asparaginase from Bacillus licheniformis by the Statistical optimization of Three-Phase Partitioning system. International Journal Pharmaceutical and Clinical Research. (2015), 7(2): 144-147.
19. Kumari, K., Biswas, T., Singh, H. R., Jha, S. K., Screening of Medium Components for The      Enhanced Production of Pseudomonas fluorescens L-Glutaminase by Plackett Burman Design. International Journal Advanced Research. (2015), 3(1): 177-185
20. Sinha, R. K., Singh, H. R., Jha, S. K., Production, Purification and Kinetic Characterization of L-Asparaginase from Pseudomonas fluorescens. International Journal of Pharmacy and Pharmaceutical Sciences. (2015),7(1):135-138.

             2014

21. Jha, S. K., Singh, H.R., Nigam, V. K., Vidyarthi, A. S., Optimization of recombinant L-asparaginase purification by Escherichia coli K-12 using three phase partitioning system by Taguchi design of experiment. Journal of Chemical Pharmaceutical Research. (2014), 6(12):839-843.
22. Jha, S. K., Pasrija, D., Singh, H. R, Nigam, V. K., Vidyarthi, A. S.,Batch Production and Media Optimization of Anti-leukemic L-Asparaginase from Pseudomonas Fluorescens by Taguchi DOE Methodology. Journal of Applied Pharmaceutical Science. (2014), 4 (12):085-089. (SCOPUS, IF: 0.26)
23. Jha, S. K., Kumari, M., Singh, H.R., Nigam, V. K., Vidyarthi, A. S., Optimization of solid-state production of antileukemic enzyme (L-asparaginase) by E. Coli K-12 using Taguchi Doe and three phase-based recovery. Int. J. Current Microbiol. Appl. Sc. (2014), 3 (9): 446-453.
24. Biswas, T., Kumari, K., Singh, H. R., Jha, S. K., Optimization of Three-Phase Partitioning system for Enhanced Recovery of L-Asparaginase from Escherichia Coli K12 Using Design of Experiment (DOE). International Journal of Advanced Research. (2014), 2(5):1142-1147.
25. Jha, S. K., Rai, A., Singh, H. R., High Throughput Screening of Potential Inhibitors of Alzheimer’s Disease Associated Tau Aggregation Protein. International Journal of Pharmaceutical Research. (2014), 6 (2), 61-63.
26. Jha, S. K., Rashmi S,Shubhra., Singh, H. R., (2014). High Throughput Screening of Quorum Sensing Inhibitors Based Lead Molecules for Pseudomonas Aeruginosa Associated Infections. International Journal of Pharmaceutical and Clinical Research. (2014), 6(3): 214-220.
27. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S. K., Effect of IVNT on Ovarian Weight, Follicle Number, Estrus Frequency and Reversibility of Reproductive Cycle in Albino Rat. International Journal of Pharmaceutical Sciences and Research, (2014), 5(8): 3293-3296
28. Parashar P, Dubey A.K, Jha, S. K, Singh. H.R, Effect of Anti-Kala-zar drug Miltefosine on ovarian Ascorbic acid Level in Albino Rats. International Journal Advanced Research, (2014), 2(5) 1148-1150.

2013

29. Jha, S. K., Pasrija, D., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., Enhanced recovery of L-Asparaginase by the Optimization of Three Phase Partitioning system by Taguchi DOE Methodology. BioProcessing. Journal. (2013), 38-42.
30. Mahto, P. K., Dubey, A. K., Parashar, P., Singh, H. R., Jha, S. K., (2013) Effect of O. sanctum (L) Leaf Extract on Testicular Tissue Ascorbic Acid in Arsenite Exposed Albino Rats, The Pharma Review. May-June (2013), pp 120-122.
31. Sinha, R., Singh, H. R., Jha, S. K., Microbial L-Asparaginase: Present And Future Prospective, International Journal of Innovative Research in Science, Engineering and Technology. (2013), 2(11):7031-7051.

             2012 & Earlier

32. Jha, S. K., Pasrija, D., Sinha, R. K., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., Microbial L-Asparaginase: A Review on Current Scenario And Future Prospects. International Journal of Pharmaceutical Science and Research. (2012), 3(9):3076-3090.
33. P. Parashar, S. K. Singh, A. K. Dubey, H. R. Singh, G. N. Trivedi, Changes in Salivary Seminal Ascorbic acid level in men of different body mass index (BMI) range groups, J. Exp. Zool. India, (2011), 14(1):249-251.
34. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S.K., Vidyarthi, A.S., Effect of Intravaginal Neem Oil Treatment on Uterine Total Phosphorus Level In Albino Rats, J. Exp. Zool. India. (2009),12(2):391-393.)
35. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S.K., Vidyarthi, A.S., Study of Ovarian and Uterine Ascorbic Acid Level after Intravaginal Neem Oil treatment in Albino rat: PHARMBIT. (2009), 20(2):154-157.
36. Singh, H.R., Singh, R.R., Singh, C.K., Dubey, A.K. Effects of intravaginal neem oil treatment on uterine glucose level in albino rats. J. Exp. Zool. India, (1998), 1(1):55-59.

1. Life Member of Association of Microbiologist of India. 2440-2008
2. Life Member of Society for Reproductive Biology and Comparative Endocrinology. LM295-2010
3. Life Member of Biotech Research Society of India (BRSI) LM 963 -2021
4. Life member of the Indian Society for the Technical Education. LM 63115 -2009

1. Prakash Pragya, Shoaib Haidar, and Hare Ram Singh. "Addressing Challenges in Cell Lysis: Effective Strategies and Technologies." (2025). Intech Open Series Biochemistry, Volume 63; DOI: 10.5772/intechopen.1008313 (ISSN: 2632-0983)
2. Prakash P.;Pandey S.;Jha S.K.;Singh H.R, Development of bioreactors: current scenario and future challenges, Techno-economics and Life Cycle Assessment of Bioreactors: Post-COVID-19 Waste Management Approach, (2022),  37-54. https://doi.org/10.1016/B978-0-323-89848-5.00012-3, ELSEVIER (ISBN: 978-0-323-89848-5)
3. Naha A.;Roy D.;Singh H.R.;Jha S.K, Phytogenic synthesis of silver nanoparticles: mechanisms and applications, Nanobiotechnology: Microbes and Plant Assisted Synthesis of Nanoparticles, Mechanisms and Applications, (2021), 167-185. https://doi.org/10.1016/B978-0-12-822878-4.00011-0, ELSEVIER (ISBN: 978-0-12-822878-4)
4. Naha A.; Saha S.;Singh H.R.;Shukla S.K.;Tripathi V.K.;Jha S.K, Recent trends and future perspectives in applications of biofiltration, An Innovative Role of Biofiltration in Wastewater Treatment Plants (WWTPs), ( 2021),  113-136. https://doi.org/10.1016/B978-0-12-823946-9.00024-3, ELSEVIER (ISBN: 978-0-12-823946-9)
5. Saha S.;Shukla S.K.;Singh H.R.;Singh B.;Jha S.K, Bioactive compounds from microalgae, An Integration of Phycoremediation Processes in Wastewater Treatment, (2021), 337-358. https://doi.org/10.1016/B978-0-12-823499-0.00005-5, ELSEVIER, (ISBN: 978-0-12-823499-0)
6. Jha S.K.;Singh H.R.;Prakash P.;Fatimah F, Nanobiotechnological interventions for the removal of toxic pollutants, Removal of Toxic Pollutants through Microbiological and Tertiary Treatment: New Perspectives, ( 2020), 335-347. https://doi.org/10.1016/B978-0-12-821014-7.00013-7, ELSEVIER (ISBN: 978-0-12-821014-7)
7. Jha S.;Singh H.;Sinha R.;Prakash P, In silico modelling of hepatocellular carcinoma linked PARP-1 protein and screening of potential inhibitors, Applications of Biotechnology for Sustainable Development,  (2017),  157-168. https://link.springer.com/chapter/10.1007/978-981-10-5538-6_19, SPRINGER (ISBN: 978-981-10-5537-9)
8. Jha S.;Singh H.;Prakash P, Dietary Fiber and Human Health: An Introduction, Dietary Fiber for the Prevention of Cardiovascular Disease: Fiber's Interaction between Gut Micoflora, Sugar Metabolism, Weight Control and Cardiovascular Health,  (2017),  1-22. https://doi.org/10.1016/B978-0-12-805130-6.00001-X, Publisher ELSEVIER (ISBN: 9780128051306).
9. D. Prasad, H.R. Singh, D.M. Pandey, A.B. Prasad and A.S. Vidyarthi, BIOTECHNOLOGICAL INTERVENTIONS FOR SUSTAINABLE RURAL DEVELOPMENT AND UPLIFTMENT, Biotechnology for Sustainable Development, (2010), 101-127, Tata Mc. Graw Hill, New Delhi. (ISBN:978-0-07-070832-7).

List of Patents (Only Published or Granted)