Birla Institute of Technology, Mesra
Dr. Hare Ram Singh
Assistant Professor, Bioengineering and Biotechnology
M.Sc., Ph.D. 		Contact Address
Permanent Address M.P Bagh, Ara, Bhojpur, Bihar
Local Address C-II/44, BIT Mesra, Ranchi, Jharkhand, India Pin:835215
Phone (Office) 9430220114
Phone Residence 9430220114
Email Id hrsingh@bitmesra.ac.in
Joined Institute on : 2-Oct-2007
  Work Experience
 
Teaching : 15 Years

Research : 19 Years

Individual: 15 Years
  Research Areas
 
  1. Production of Biotherapeutics
  2. Development of Nanobiotics
  3. Process Development for the Production of Commercially Important Enzymes.
  Publications
 
  1. Pragya Prakash, Sanjeev Chandrayan, Purnima Tiwari, Hare Ram Singh, Santosh Kumar Jha, Development of a downstream process for purification and purity analysis of glutaminase free L-asparaginase using UPLC, DLS-ZP and DSCTGA, Journal of Taibah University for Science, 15:1, (2021), 458-467, DOI: 10.1080/16583655.2021.1984694.
  2. Farkhunda Fatima, Hare Ram Singh, Santosh Kumar Jha, Assessment of antioxidant and cytotoxicity activities against A 549 lung cancer cell line by synthesized reduced graphene oxide nanoparticles mediated by Camellia sinensis, 3 Biotech (2021)  11:494.
  3. Ananya Naha, Santosh Kumar Jha, Hare Ram Singh, Muthu Kumar Sampath. Kinetic modelling and statistical optimization of submerged production of anti-Parkinson’s prodrug L-DOPA by Pseudomonas fluorescens, Preparative Biochemistry and Biotechnology, (2021), https://doi.org/10.1080/10826068.2021.1945624.
  4. Sushil Kumar Shukla, Sneha Pandey, Swastika Saha, Hare Ram Singh, Pradeep Kumar Mishra, Sunil Kumar, Santosh Kumar Jha, Removal of crystal violet by Cu-chitosan nano-biocomposite particles using Box–Behnken design, Journal of Environmental Chemical Engineering 9 (2021), 105847.
  5. Pragya Prakash, Hare Ram Singh, Santosh Kumar Jha. Preparation, Characterization and Application of Curcumin-based Polymeric Bio-composite for Efficient Removal of Endotoxins and Bacterial Cells from Therapeutic Preparations. Journal of Biomaterials Science: Polymer Edition, 2020 (SCI, IF: 2.69).
  6. Farkhunda Fatima, Hare Ram Singh, Santosh Kumar Jha. Camellia sinesis mediated green synthesis of reduced graphene oxide nanoparticles: Evaluation of antioxidant and cytotoxicity. Journal of Biomaterials Science: Polymer Edition, 2020 (Submitted).
  7. Prakash P, Singh HR, Jha SK. Indicator dye based screening of glutaminase free L-asparaginase producer and kinetic evaluation of enzyme production process. Preparative biochemistry & biotechnology, 2020. https://doi.org/10.1080/10826068.2020.1737942 (SCI, IF: 1.415)
  8. S. Saha, K.K. Pradhan, S.K. Shukla, H.R. Singh, S.K. Jha. Production and purification of bioflocculants from newly isolated bacterial species: A comparative decolorization study of cationic and anionic textile dyes. Environmental Technology, 2020. 15:1-12. https://doi.org/10.1080/09593330.2020.1737737. (SCI, IF: 2.13)
  9. Prakash P, Singh HR, Jha SK. Production, purification and kinetic characterization of glutaminase free anti-leukemic L-asparaginase with low endotoxin level from novel soil isolate. Preparative biochemistry & biotechnology, 2019; 23, 1-12. (SCI, IF: 1.415
  10. Naha A., Singh H.R., Jha S.K. L-DOPA a Promising Pro-Drug against Parkinson Disease: Present and Future Perspective. Research Journal of Biotechnology, 2019; 14 (9), 131-139.
  11. Sinha R., Singh H.R, Cloning, purification and kinetic characterization of
    L-asparaginase from Pseudomonas fluorescens. Research Journal of Biotechnology, 2019; 14 (12), 104-110.
  12. S Nezami, K Joseph, A Ahsan, LVS Aparna, SR Sharma, SK Jha. Screening of commercially important halophilic microbial community for bioactive compounds. International Journal of Pharmaceutical, Chemical & Biological Sciences, 2016; 6 (3):309-321.
  13. Parasar P, Dubey, A.K., Singh H.R., Jha S.K. Changes in Rheological and Biochemical Properties of Saliva and Semen in Relation to Increasing Body Mass Index (BMI) of Human Subjects. International Journal of Pharmaceutical Sciences Review and Research, 2016; 37(1):122-124.
  14. Perween, Q., Anand, V., Singh, H. R., Jha, S. K., Enhanced Recovery of L-glutaminase by The Optimization of A Three-phase Partitioning System Using The Taguchi Doe Methodology. Int. J. Pharma. Clinical Res. 2015, 7(3): 216-220.
  15. Anand, V., Perween, Q., Sinha, R. K., Singh, H. R., Jha, S. K., Screening of L-glutaminase Producer   and Optimization of Production Process by Taguchi Orthogonal Array. Int. J. Pharma. Clinical Res. 2015, 7(4): 246-251.
  16. Jha, S. K., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., A Heuristic Approach for the enhanced recovery of anti-leukemic L-Asparaginase from Bacillus licheniformis by the Statistical optimization of Three-Phase Partitioning system. Int. J. Pharma. Clinical Res. 2015, 7(2): 144-147.
  17. Kumari, K., Biswas, T., Singh, H. R., Jha, S. K., Screening of Medium Components for The      Enhanced Production of Pseudomonas fluorescens L-Glutaminase by Plackett Burman Design. Int. J. Advance Res. 2015, 3(1): 177-185.
  18. Jha, S. K., Singh, H.R., Nigam, V. K., Vidyarthi, A. S., Optimization of recombinant L-asparaginase purification by Escherichia coli K-12 using three phase partitioning system by Taguchi design of experiment. J. Chem. Pharma. Res. 2014, 6(12):839-843.
  19. Jha, S. K., Sinha, R. K., Singh, H. R., Production, Purification and Kinetic Characterization of L-Asparaginase from Pseudomonas fluorescens. Int. J. Pharma. Pharmaceut. Sci. 2014,7(1):135-138.
  20. Jha, S. K., Pasrija, D., Singh, H. R.,Batch Production and Media Optimization of Anti-leukemic L-Asparaginase from Pseudomonas Fluorescens by Taguchi DOE Methodology. Journal of Applied Pharmaceutical Science. 2014, 4 (12):085-089.
  21. Jha, S. K., Kumari, M., Singh, H.R., Nigam, V. K., Vidyarthi, A. S., Optimization of solid state production of antileukemic enzyme (L-asparaginase) by E. Coli K-12 using Taguchi Doe and three phase based recovery. Int. J. Current Microbiol. Appl. Sc. 2014, 3 (9): 446-453.
  22. Biswas, T., Kumari, K., Singh, H. R., Jha, S. K., Optimization of Three-Phase Partitioning system for Enhanced Recovery of L-Asparaginase from Escherichia Coli K12 Using Design of Experiment (DOE). Int. J. Adv. Res. 2014, 2(5):1142-1147
  23. Jha, S. K., Rai, A., Singh, H. R., High Throughput Screening of Potential Inhibitors of Alzheimer’s Disease Associated Tau Aggregation Protein. Int. J. Pharma. Res. 2014, 6 (2), 62-66.
  24. Jha, S. K., Rashmi S.S., Singh, H. R., (2014). High Throughput Screening of Quorum Sensing Inhibitors Based Lead Molecules for Pseudomonas Aeruginosa Associated Infections. Int. J. Pharma. Clinical Res. 2014, 6(3): 214-220.
  25. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S. K., Effect of IVNT on Ovarian Weight, Follicle Number, Estrus Frequency and Reversibility of Reproductive Cycle in Albino Rat. Int. J.Pharma. Sc. Res, 2014, 5(8): 3293-3296.
  26. Parashar, Dubey A.K, Jha, S. K, Singh. H.R, Effect of Anti-Kala-zar drug Miltefosine on ovarian Ascorbic acid Level in Albino Rats. Int. J. Adv. Sc. Res, 2014, 2(5) 1148-1150
  27. Jha, S. K., Pasrija, D., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., Enhanced recovery of L-Asparaginase by the Optimization of Three Phase Partitioning system by Taguchi DOE Methodology. BioProcess. J. 2013, 11: 40-45.
  28. Mahto, P. K., Dubey, A. K., Parashar, P., Singh, H. R., Jha, S. K., (2013) Effect of O. sanctum (L) Leaf Extract on Testicular Tissue Ascorbic Acid in Arsenite Exposed Albino Rats, The Pharma Review. May-June 2013, pp 120-122.
  29. Sinha, R., Singh, H. R., Jha, S. K. (2013) Microbial L-Asparaginase: Present And Future Prospective, Int. J. Innov. Res. Sci. Eng. Technol.2013, 2(11):7031-7051.
  30. Jha, S. K., Pasrija, D., Sinha, R. K., Singh, H. R., Nigam, V. K., Vidyarthi, A. S., Microbial L-Asparaginase: A Review On Current Scenario And Future Prospects. Int. J. Pharma. Sci. Res. 2012, 3(9):3076-3090.
  31. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S.K., Vidyarthi, A.S., Effect of Intravaginal Neem Oil Treatment on Uterine Total Phosphorus Level In Albino Rats, J. Exp. Zool. India. 2009,12(2):391-393.
  32. P. Parashar, S. K. Singh, A. K. Dubey, H. R. Singh, G. N. Trivedi, Changes in Salivary Seminal Ascorbic acid level in men of different body mass index (BMI) range groups, J. Exp. Zool. India, 2011, 14(1):249-251.
  33. Singh, H. R., Singh, R. R., Dubey, A. K., Jha, S.K., Vidyarthi, A.S., Study of Ovarian and Uterine Ascorbic Acid Level after Intravaginal Neem Oil treatment in Albino rat: PHARMBIT. 2009, 20(2):154-157.
  34. Singh, H.R., Singh, R.R., Singh, C.K., Dubey, A.K. Effects of intravaginal neem oil treatment on uterine glucose level in albino rats. J. Exp. Zool. India, 1998, 1(1):55-59.
  35. Singh, H.R., Singh, R.R., Singh, C.K., Dubey, A.K. Effects of intravaginal neem oil treatment on uterine protein level in albino rats. Trends in Lifesciences (India), 1998, 13(1):1-3.

 
  Member of Professional Bodies
 
  1. Life Member of Association of Microbiologist of India.
  2. Life Member of Society for Reproductive Biology and Comparative Endocrinology.
  3. Life Member of Biotech Research Society of India (BRSI)
  4. Associate member of the Indian Society for the Technical Education
  Current Sponsored Projects
 

S.N.

Title of Project

Funding Agency

Duration

Grant mobilized

(Rs. lakhs)

Principal Investigator

(PI)

Status:

 

from

to
  Development of Novel Antileukemics Through the structural and Functional Studies of Engineered and kimeric Plant-Type Asparaginases  SERB(DST) Govt. of India. 08.02.2022    33.66264 Co-PI On going

1.

Development of Android mobile Based System for Monitoring of Plant Pathogen Infection Using Foldscope and Knowledge Dissemination in Aspirational districts.

DBT, GoI

 

 

06/04/2018

05/09/2019

8L+10.12L=18.12

Co-PI

Completed

2.

Process development for the production of UV-protective, antioxidative and antimicrobial pigments from bacterial source using dairy waste.

BIRAC, DBT, GoI

 

04/04/2017

03/09/2018

46.30

Co-PI

Completed

3.

Development of extractive fermentation methodology for the enhanced production of endotoxin free L-asparaginase.

BIT-SMS

 

13/11/2015

12/11/2016

0.80

Co-PI

Completed

 

 

 

 

 

 

 

 
  Text and Reference Books
 
  1. Biotechnology for Rural Development: Biotechnological Interventions for Sustainable Rural Development and upliftment.101-127 Publisher: Tata Mc. Graw Hill, New Delhi. ISBN:978-0-07-070832-7.
  2. Dietary fiber for the prevention of cardiovascular disease: Why fiber matters? ELSEVIER, (ISBN: 9780128051306).
  3. Application of biotechnology for sustainable development: Computational Modelling of Hepatocellular Carcinoma Associated PARP-1 protein And Structure Base Screening of Potential Inhibitor. SPRINGER (ISBN 978-981-10-5537-9).
  4. Nano-biotechnological interventions for Removal of Toxic Pollutants. 2020, ELSEVIER (ISBN: 978-0-12-821014-7)
  Intellectual Property Rights (IPR)
 
  1. Patent Filed on Production of Microbial Pigment (Prodigiosin) by using Dairy Waste (Ref No: 201931011066).